Antiserum Against Tyrosinase from Mouse Melanoma
نویسندگان
چکیده
منابع مشابه
Protein specific N-glycosylation of tyrosinase and tyrosinase-related protein-1 in B16 mouse melanoma cells.
Tyrosinase and tyrosinase-related protein-1 (TRP-1) are two melanogenic enzymes that regulate melanin biosynthesis. Both are glycoproteins and belong to the TRP-1 gene family. They share a significant level of sequence similarity in several regions, including the catalytic domain and the potential N-glycosylation sites. We have recently shown that inhibition of the early steps of N-glycan proce...
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The antiserum against mouse growth hormone (MGH) was produced by immunizing monkeys and some basic immunological properties of the antiserum were studied. The antiserum formed single precipitin lines in agar gel double diffusion plate against both MGH preparation and the crude extract of mouse anterior pituitaries, but reacted with neither normal mouse serum nor the crude extracts of several vi...
متن کاملSeparation and characterization of multiple forms of tyrosinase from mouse melanoma.
The soluble and insoluble (particle-bound) tyrosinase (EC 1.10.3.1) present in extracts of Harding-Passey, B-16, and Cloudman S-91 mouse melanoma was examined by means of differential solubility, continuous-flow paper electrophoresis and acrylamide-gel electrophoresis. Two soluble tyrosinases (T1 and T2) were found to exist as distinct entities in each type of melanoma examined. The rela tive c...
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Tyrosinase activity was demonstrated in the sera from melanoma-bearing mice. One, two, and three weeks following tumor transplantation, 12, 80, and 90% of the respective sera from these mice showed positive results. No sera from normal mice had tyrosinase activity. There was a correlation between tumor size and tyrosinase activity, the coefficient of correlation being 0.71. The activity in the ...
متن کاملDetection of tyrosinase mRNA from the blood of melanoma patients.
Surgical therapy for localized melanoma is highly successful. However, if melanoma spreads beyond its primary site, the results of treatment are poor. Therefore, early detection of circulating melanoma cells in the blood may be important. Currently, circulating melanoma cells are undetectable. Tyrosinase is an enzyme in the melanin synthetic pathway the expression of which is only found in mela...
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ژورنال
عنوان ژورنال: Journal of Investigative Dermatology
سال: 1972
ISSN: 0022-202X
DOI: 10.1111/1523-1747.ep12539941